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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SBARDELLA, A. P.; WELLER, M. M. D. C. A.; I. FONSECA; N. B. STAFUZZA; P. A. BERNARDES; F. F. e SILVA; SILVA, M. V. G. B.; MARTINS, M. F.; D. P. MUNARI. |
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Afiliação: |
A. P. SBARDELLA; M. M. D. C. A. WELLER; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Ribonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 102, n. 2, p. 1761-1767, 2019. |
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DOI: |
https://doi.org/10.3168/jds.2018-15516 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation. |
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Palavras-Chave: |
BaySeq; Crossbred dairy cow; Cuffdiff 2; EdgeR; RNA sequencing. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02292naa a2200289 a 4500
001 2117886
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15516$2DOI
100 1 $aSBARDELLA, A. P.
245 $aRibonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae.$h[electronic resource]
260 $c2019
520 $aThe aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation.
653 $aBaySeq
653 $aCrossbred dairy cow
653 $aCuffdiff 2
653 $aEdgeR
653 $aRNA sequencing
700 1 $aWELLER, M. M. D. C. A.
700 1 $aI. FONSECA
700 1 $aN. B. STAFUZZA
700 1 $aP. A. BERNARDES
700 1 $aF. F. e SILVA
700 1 $aSILVA, M. V. G. B.
700 1 $aMARTINS, M. F.
700 1 $aD. P. MUNARI
773 $tJournal of Dairy Science$gv. 102, n. 2, p. 1761-1767, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 8 | |
| 1. |  | MARCHESI, J. P.; SBARDELLA, A. P.; JOAQUIM, L. B.; CANTAO, M. E.; PEIXOTO, J. de O.; BUZANKAS, M. E.; LEDUR, M. C.; MUNARI, D. P. Linkage disequilibrium and effective population size in a paternal broiler line. In: INTERNATIONAL MEETING OF ADVANCES IN ANIMAL SCIENCE, 2016, Jaboticabal. Posters presentations... Jabotical: PPGZ Unesp, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SBARDELLA, A. P; FONSECA, I.; WELLER, M. A. DEL C. A.; STAFUZZA, N. B.; OLIVEIRA, J. R.; WATANABE, R. N.; COSTA, R, M. da; CARVAJAL, A. B.; SILVA, M. V. G. B.; MARTINS, M. F.; MUNARI, D. P. Avaliação de abordagens estatísticas para análise da expressão gênica diferencial em dados reais de RNA-seq In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto. Anais... Ribeirão Preto: SBMA, 2017. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | SBARDELLA, A. P.; FONSECA, I.; WELLER, M. M. D. C. A.; STAFUZZA, N. B.; OLIVEIRA, J. R.; WATANABE, R. N.; COSTA, R. M. da; CARVAJAL, A. B.; SILVA, M. V. G. B.; MARTINS, M. F.; MUNARI, D. P. Avaliação de abordagens estatísticas para análise da expressão gênica diferencial em dados reais de RNA-Seq. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 12., 2017, Ribeirão Preto. Anais... Ribeirão Preto: SBMA, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | PIRES, B. C.; THOLON, P.; BUZANSKAS, M. E.; SBARDELLA, A. P.; ROSA, J. O.; SILVA, L. O. C. da; TORRES JUNIOR, R. A. de A.; MUNARI, D. P.; ALENCAR, M. M. de. Genetic analyses on bodyweight, reproductive, and carcass traits in composite beef cattle. Animal Production Science, v. 57, n. 3, 415-421, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 5. | | PEREIRA, H. P.; VERARDO, L. L.; WELLER, M. M. D. C. A.; SBARDELLA, A. P.; MUNARI, D. P.; DAIBERT, R. M. de P.; CARVALHO, W. A.; MACHADO, M. A.; MARTINS, M. F. Going further post-RNA-seq: in silico functional analyses revealing candidate genes and regulatory elements related to mastitis in dairy cattle. Journal of Dairy Research, v. 88, p. 286-292, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | MARCHESI, J. A. P.; BUZANSKAS, M. E.; CANTAO, M. E.; IBELLI, A. M. G.; PEIXOTO, J. de O.; JOAQUIM, L. B.; MOREIRA, G. C. M.; GODOY, T. F.; SBARDELLA, A. P.; FIGUEIREDO, E. A. P. de; COUTINHO, L. L.; MUNARI, D. P.; LEDUR, M. C. Relationship of runs of homozygosity with adaptive and production traits in a paternal broiler line. Animal, v. 12, n. 6, p. 1126-1134, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. Isolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae. Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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